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dc.contributor.authorOgunkolade, B. William
dc.contributor.authorAdaikalakoteswari, Antonysunil
dc.contributor.authorCardoso, Shirleny Romualdo
dc.contributor.authorLowe, Rob
dc.contributor.authorPatel, Nisha
dc.contributor.authorRakyan, Vardhman
dc.contributor.authorFiner, Sarah
dc.contributor.authorWabitsch, Martin
dc.contributor.authorSaravanan, Ponnusamy
dc.contributor.authorTripathi, Gyanendra
dc.contributor.authorBochukova, Elena
dc.contributor.authorHitman, Graham A
dc.identifier.citationOgunkolade, B.W., Adaikalakoteswari, A., Cardoso, S.R., Lowe, R., Patel, N., Rakyan, V., Finer, S., Wabitsch, M., Saravanan, P., Tripathi, G. and Bochukova, E., (2021). 'An integrative epi-transcriptomic approach identifies the human cartilage chitinase 3-like protein 2 (CHI3L2) as a potential mediator of B12 deficiency in adipocytes'. Epigenetics, pp. 1-15.en_US
dc.description.abstractVitamin B12 has multiple biochemical functions including in the one-carbon cycle generating a methyl group for DNA methylation, and metabolism of fatty acids and amino acids to generate energy via the citric acid cycle. The aim of our study was to use a combined epigenomic and transcriptomic approach to identify novel genes mediating the effect of B12 on adipogenesis. Human pre-adipocytes (CHUB-S7) were treated with a range of B12 (0–500 nM) concentrations from the day of cell seeding until harvesting in discovery and validation experiments prior to genome-wide methylation analysis using the Illumina HumanMethylation 450Beadchip. For transcriptomic analysis, RNA-seq libraries were run on the Illumina HiSeq 2500. To further investigate the expression of any genes on human adipogenesis, a second human preadipocyte strain was studied (SGBS) by real-time quantitative PCR (qRT-PCR). A combined epigenetic and transcriptomic approach in differentiated human pre-adipocyte cell line, CHUB-S7, identified that the Human cartilage chitinase 3-like protein 2 (CHI3L2) gene was hypo-methylated and had increased expression in low B12 conditions. Furthermore, there was an approximately 1000-fold increase in CHI3L2 expression in the early days of adipocyte differentiation, which paralleled an increase of lipid droplets in differentiated SGBS cells and an increased expression level of markers of mature adipocytes. In summary, we have identified a potential role of the human cartilage chitinase 3-like protein 2 (CHI3L2) in adipocyte function in the presence of low B12 levels.en_US
dc.description.sponsorshipQueen Mary University of Londonen_US
dc.publisherInforma UK Limiteden_US
dc.subjectCancer Researchen_US
dc.subjectMolecular Biologyen_US
dc.titleAn integrative epi-transcriptomic approach identifies the human cartilage chitinase 3-like protein 2 (CHI3L2) as a potential mediator of B12 deficiency in adipocytesen_US
dc.contributor.departmentUniversity of Derbyen_US

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