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dc.contributor.authorTroth, Christopher R.
dc.contributor.authorSweet, Michael J.
dc.contributor.authorNightingale, Jen
dc.contributor.authorBurian, Alfred
dc.date.accessioned2021-02-01T10:57:11Z
dc.date.available2021-02-01T10:57:11Z
dc.date.issued2021-01-06
dc.identifier.citationTroth, C.R., Sweet, M.J., Nightingale, J. and Burian, A., (2021). 'Seasonality, DNA degradation and spatial heterogeneity as drivers of eDNA detection dynamics'. Science of The Total Environment, pp. 1-8.en_US
dc.identifier.issn0048-9697
dc.identifier.doi10.1016/j.scitotenv.2020.144466
dc.identifier.urihttp://hdl.handle.net/10545/625573
dc.description.abstractIn recent years, eDNA-based assessments have evolved as valuable tools for research and conservation. Most eDNA-based applications rely on comparisons across time or space. However, temporal, and spatial dynamics of eDNA concentrations are shaped by various drivers that can affect the reliability of such comparative approaches. Here, we assessed (i) seasonal variability, (ii) degradation rates and (iii) micro-habitat heterogeneity of eDNA concentrations as key factors likely to inflict uncertainty in across site and time comparisons. In a controlled mesocosm experiment, using the white-clawed crayfish as a model organism, we found detection probabilities of technical replicates to vary substantially and range from as little as 20 to upwards of 80% between seasons. Further, degradation rates of crayfish eDNA were low and target eDNA was still detectable 14–21 days after the removal of crayfish. Finally, we recorded substantial small-scale in-situ heterogeneity and large variability among sampling sites in a single pond of merely 1000m2 in size. Consequently, all three tested drivers of spatial and temporal variation have the potential to severely impact the reliability of eDNA-based site comparisons and need to be accounted for in sampling design and data analysis of field-based applications.en_US
dc.description.sponsorshipSurescreenen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S0048969720379973?via=ihuben_US
dc.rights© 2021 Elsevier B.V. All rights reserved.
dc.rightsCC0 1.0 Universal*
dc.rights.urihttps://www.elsevier.com/tdm/userlicense/1.0/
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.subjecteDNAen_US
dc.subjectwhite clawed crayfishen_US
dc.subjectseasonalityen_US
dc.subjectqPCRen_US
dc.titleSeasonality, DNA degradation and spatial heterogeneity as drivers of eDNA detection dynamicsen_US
dc.typeArticleen_US
dc.contributor.departmentUniversity of Derbyen_US
dc.contributor.departmentSureScreen Scientifics Ltd, Morleyen_US
dc.contributor.departmentBristol Zoological Society, Clifton, Bristolen_US
dc.contributor.departmentUniversity of Bristolen_US
dc.contributor.departmentLurio University, Nampula, Mozambiqueen_US
dc.identifier.journalScience of the Total Environmenten_US
dc.identifier.piiS0048969720379973
dc.source.journaltitleScience of The Total Environment
dc.source.volume768
dc.source.beginpage144466
dcterms.dateAccepted2020-12-07
dc.author.detail783337en_US


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