• Login
    View Item 
    •   Home
    • Research Publications
    • Research Centres & Groups
    • Environmental Sustainability Research Centre
    • View Item
    •   Home
    • Research Publications
    • Research Centres & Groups
    • Environmental Sustainability Research Centre
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UDORACommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    About and further information

    AboutOpen Access WebpagesOpen Access PolicyTake Down Policy University Privacy NoticeUniversity NewsTools for ResearchersLibraryUDo

    Statistics

    Display statistics

    Reliable eDNA detection and quantification of the European weather loach (Misgurnus fossilis)

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Brys, Rein
    Halfmaerten, David
    Neyrinck, Sabrina
    Mauvisseau, Quentin
    Auwerx, Johan
    Sweet, Michael
    Mergeay, Joachim
    Affiliation
    Research Institute for Nature and Forest, Geraardsbergen, Belgium
    University of Derby
    SureScreen Scientifics Ltd
    Issue Date
    2020-03-10
    
    Metadata
    Show full item record
    Abstract
    The European weather loach (Misgurnus fossilis) is a cryptic and poorly known fish species of high conservation concern. The species is experiencing dramatic population collapses across its native range to the point of regional extinction. Although environmental DNA (eDNA)‐based approaches offer clear advantages over conventional field methods for monitoring rare and endangered species, accurate detection and quantification remain difficult and quality assessment is often poorly incorporated. In this study, we developed and validated a novel digital droplet PCR (ddPCR) eDNA‐based method for reliable detection and quantification, which allows accurate monitoring of M. fossilis across a number of habitat types. A dilution experiment under laboratory conditions allowed the definition of the limit of detection (LOD) and the limit of quantification (LOQ), which were set at concentrations of 0.07 and 0.14 copies μl–1, respectively. A series of aquarium experiments revealed a significant and positive relationship between the number of individuals and the eDNA concentration measured. During a 3 year survey (2017–2019), we assessed 96 locations for the presence of M. fossilis in Flanders (Belgium). eDNA analyses on these samples highlighted 45% positive detections of the species. On the basis of the eDNA concentration per litre of water, only 12 sites appeared to harbour relatively dense populations. The other 31 sites gave a relatively weak positive signal that was typically situated below the LOQ. Combining sample‐specific estimates of effective DNA quantity (Qe) and conventional field sampling, we concluded that each of these weak positive sites still likely harboured the species and therefore they do not represent false positives. Further, only seven of the classified negative samples warrant additional sampling as our analyses identified a substantial risk of false‐negative detections (i.e., type II errors) at these locations. Finally, we illustrated that ddPCR outcompetes conventional qPCR analyses, especially when target DNA concentrations are critically low, which could be attributed to a reduced sensitivity of ddPCR to inhibition effects, higher sample concentrations being accommodated and higher sensitivity obtained.
    Citation
    Brys, R., Halfmaerten, D., Neyrinck, S., Mauvisseau, Q., Auwerx, J., Sweet, M. and Mergeay, J., (2020).'Reliable eDNA detection and quantification of the European weather loach (Misgurnus fossilis)'. Journal of Fish Biology, pp. 1-16.
    Publisher
    Wiley
    Journal
    Journal of Fish Biology
    URI
    http://hdl.handle.net/10545/624683
    DOI
    10.1111/jfb.14315
    Additional Links
    https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfb.14315
    Type
    Article
    Language
    en
    ISSN
    0022-1112
    EISSN
    1095-8649
    ae974a485f413a2113503eed53cd6c53
    10.1111/jfb.14315
    Scopus Count
    Collections
    Environmental Sustainability Research Centre

    entitlement

     
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.