Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP‐1 secretory activity in vitro and acute glucose lowering effects in mice
Authors
Parthsarathy, VadivelMclaughlin, Christopher
Harnedy, Padraigin
Allsopp, Phillip
Crowe, William
McSorley, Emeir
FitzGerald, Dick
O'Harte, Finbarr
Issue Date
2018-10-16
Metadata
Show full item recordAbstract
The anti‐diabetic actions of a boarfish protein hydrolysate (BPH) were investigated in cultured cells and mice. A boarfish (Capros aper) muscle protein hydrolysate was generated using the enzymes Alcalase 2.4 L and Flavourzyme 500 L. Furthermore, the BPH was subjected to simulated gastrointestinal digestion (SGID). BPH and SGID samples (0.01–2.5 mg mL−1) were tested in vitro for DPP‐IV inhibition and insulin and GLP‐1 secretory activity from BRIN‐BD11 and GLUTag cells, respectively. The BPH and SGID samples, caused a dose‐dependent increase (4.2 to 5.3‐fold, P < 0.001) in insulin secretion from BRIN‐BD11 cells and inhibited DPP‐IV activity (IC50 1.18 ± 0.04 and 1.21 ± 0.04 mg mL−1), respectively. The SGID sample produced a 1.3‐fold (P < 0.01) increase in GLP‐1 secretion. An oral glucose tolerance test (OGTT) was conducted in healthy mice (n = 8), with or without BPH (50 mg/kg bodyweight). BPH mediated an increase in plasma insulin levels (AUC(0–120 min), P < 0.05) and a consequent reduction in blood glucose concentration (P < 0.01), after OGTT in mice versus controls. The BPH showed potent anti‐diabetic actions in cells and improved glucose tolerance in mice.Citation
Parthsarathy, V., McLaughlin, C.M., Harnedy, P.A., Allsopp, P.J., Crowe, W., McSorley, E.M., FitzGerald, R.J. and O’Harte, F.P.M. (2018). 'Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP‐1 secretory activity in vitro and acute glucose lowering effects in mice'. International Journal of Food Science & Technology, 54 (1), pp. 271-281.Publisher
WileyJournal
International Journal of Food Science and TechnologyDOI
10.1111/ijfs.2019.54.issue-1https://doi.org/10.1111/ijfs.13975
Additional Links
http://doi.wiley.com/10.1111/ijfs.2019.54.issue-1https://pure.ulster.ac.uk/en/publications/boarfish-capros-aper-protein-hydrolysate-has-potent-insulinotropi
Type
ArticleLanguage
enISSN
09505423ae974a485f413a2113503eed53cd6c53
10.1111/ijfs.2019.54.issue-1
Scopus Count
Collections
The following license files are associated with this item:
- Creative Commons