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dc.contributor.authorGunputh, Urvashi
dc.contributor.authorLe, Huirong
dc.contributor.authorBesinis, Alexandros
dc.contributor.authorTredwin, Christopher
dc.contributor.authorHandy, Richard
dc.date.accessioned2019-10-07T13:52:10Z
dc.date.available2019-10-07T13:52:10Z
dc.date.issued2019-05-16
dc.identifier.citationGunputh, U.F., Le, H., Besinis, A., Tredwin, C. and Handy, R.D., (2019). 'Multilayered composite coatings of titanium dioxide nanotubes decorated with zinc oxide and hydroxyapatite nanoparticles: controlled release of Zn and antimicrobial properties against Staphylococcus aureus'. International journal of nanomedicine, 14, pp. 3583-3600. DOI: 10.2147/ijn.s199219en_US
dc.identifier.issn11782013
dc.identifier.doi10.2147/ijn.s199219
dc.identifier.urihttp://hdl.handle.net/10545/624192
dc.description.abstractThis study aimed to decorate the surface of TiO2 nanotubes (TiO2 NTs) grown on medical grade Ti-6Al-4V alloy with an antimicrobial layer of nano zinc oxide particles (nZnO) and then determine if the antimicrobial properties were maintained with a final layer of nano-hydroxyapatite (HA) on the composite. The additions of nZnO were attempted at three different annealing temperatures: 350, 450 and 550 °C. Of these temperatures, 350°C provided the most uniform and nanoporous coating and was selected for antimicrobial testing. The LIVE/DEAD assay showed that ZnCl2 and nZnO alone were >90% biocidal to the attached bacteria, and nZnO as a coating on the nanotubes resulted in around 70% biocidal activity. The lactate production assay agreed with the LIVE/DEAD assay. The concentrations of lactate produced by the attached bacteria on the surface of nZnO-coated TiO2 NTs and ZnO/HA-coated TiO2 NTs were 0.13±0.03 mM and 0.37±0.1 mM, respectively, which was significantly lower than that produced by the bacteria on TiO2 NTs alone, 1.09±0.30 mM (Kruskal–Wallis, P<0.05, n=6). These biochemical measurements were correlated with electron micrographs of cell morphology and cell coverage on the coatings. nZnO on TiO2 NTs was a stable and antimicrobial coating, and most of the biocidal properties remained in the presence of nano-HA on the coating.en_US
dc.description.sponsorshipPlymouth University; University of Derbyen_US
dc.language.isoenen_US
dc.publisherDove Medical Press Ltd.en_US
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529028/en_US
dc.relation.urlhttps://www.dovepress.com/multilayered-composite-coatings-of-titanium-dioxide-nanotubes-decorate-peer-reviewed-article-IJNen_US
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.subjectBiophysicsen_US
dc.subjectOrganic Chemistryen_US
dc.subjectBioengineeringen_US
dc.subjectDrug Discoveryen_US
dc.subjectBiomaterialsen_US
dc.subjectGeneral Medicineen_US
dc.titleMultilayered composite coatings of titanium dioxide nanotubes decorated with zinc oxide and hydroxyapatite nanoparticles: controlled release of Zn and antimicrobial properties against <em>Staphylococcus aureus</em></p>en_US
dc.typeArticleen_US
dc.contributor.departmentUniversity of Derbyen_US
dc.contributor.departmentPlymouth Universityen_US
dc.identifier.journalInternational Journal of Nanomedicineen_US
dc.source.volumeVolume 14
dc.source.beginpage3583-3600
dcterms.dateAccepted2019-03-21
refterms.dateFOA2019-10-07T13:52:10Z
dc.author.detail785817en_US


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