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    Identification of the splice variants of Recepteur d'Origine nantais (RON) in lung cancer cell lines

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    Authors
    Krishnaswamy, S
    Bukhari, I
    Mohammed, A.K
    Amer, O.E
    Tripathi, G
    Alokail, M.S
    Al-Daghri, N.M
    Affiliation
    University of Westminster
    Issue Date
    14/09/2018
    
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    Abstract
    RON receptor tyrosine kinase is a transmembrane protein directly involved in suppression of inflammation and its aberrant expression linked to cancers and metastasis. Efforts to block deregulated RON signaling in tumors using small molecule kinase inhibitors or antibodies have been complicated by the presence of unknown number/types of isoforms of RON, which, despite being structurally similar, localize differently and mediate varied functions. Current study was designed to identify the splice variants of RON transcripts formed by skipping of sequences between exons 9 and 14 for better understanding of isoform specific RON signaling in cancers. PCR amplification and bi-directional sequencing of a 901 bp cDNA sequence located between exons 9 to 14 of RON from lung cancer cell lines revealed the presence of two splicing variants formed by skipping of exons 11 and 11–13. Each of these transcripts was found in more than one cell line. Expressed sequence tag (EST) database search indicated that the splicing variant lacking exons 11–13 was a novel one. Here we conclude that the splice variants of RON lacking exon 11 and exons 11–13 were detected in several lung cancer cell lines. Novel variant formed by skipping exons 11–13, the sequence of which code for transmembrane region, is predicted to code for a truncated isoform that may be secreted out. Tumors may antagonize the ligand dependent anti-inflammatory function of wild-type RON by secreting out the ligand binding isoforms.
    Citation
    Krishnaswamy, S., Bukhari, I., Mohammed, A.K., Amer, O.E., Tripathi, G., Alokail, M.S. and Al-Daghri, N.M., (2018). 'Identification of the splice variants of Recepteur d'Origine nantais (RON) in lung cancer cell lines'. Gene, 679, pp.335-340. DOI: 10.1016/j.gene.2018.09.027
    Publisher
    Elsevier
    Journal
    Gene
    URI
    http://hdl.handle.net/10545/624103
    DOI
    10.1016/j.gene.2018.09.027
    Additional Links
    https://www.sciencedirect.com/science/article/pii/S0378111918309867?via%3Dihub
    Type
    Article
    Language
    en
    ISSN
    3781119
    EISSN
    18790038
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.gene.2018.09.027
    Scopus Count
    Collections
    School of Human Sciences

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