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    Surface charge visualization at viable living cells

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    Authors
    Perry, D
    Paulose Nadappuram, B
    Momotenko, D
    Voyias, P.D
    Page, A
    Tripathi, G
    Frenguelli, B.G
    Unwin, P.R
    Affiliation
    University of Warwick
    Issue Date
    12/02/2016
    
    Metadata
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    Abstract
    Scanning ion conductance microscopy (SICM) is demonstrated to be a powerful technique for quantitative nanoscale surface charge mapping of living cells. Utilizing a bias modulated (BM) scheme, in which the potential between a quasi-reference counter electrode (QRCE) in an electrolyte-filled nanopipette and a QRCE in bulk solution is modulated, it is shown that both the cell topography and the surface charge present at cellular interfaces can be measured simultaneously at high spatial resolution with dynamic potential measurements. Surface charge is elucidated by probing the properties of the diffuse double layer (DDL) at the cellular interface, and the technique is sensitive at both low-ionic strength and under typical physiological (high-ionic strength) conditions. The combination of experiments that incorporate pixel-level self-referencing (calibration) with a robust theoretical model allows for the analysis of local surface charge variations across cellular interfaces, as demonstrated on two important living systems. First, charge mapping at Zea mays root hairs shows that there is a high negative surface charge at the tip of the cell. Second, it is shown that there are distinct surface charge distributions across the surface of human adipocyte cells, whose role is the storage and regulation of lipids in mammalian systems. These are new features, not previously recognized, and their implications for the functioning of these cells are highlighted.
    Citation
    Perry, D., Paulose Nadappuram, B., Momotenko, D., et al. (2016). 'Surface charge visualization at viable living cells'. Journal of the American Chemical Society, 138(9), pp.3152-3160. DOI: 10.1021/jacs.5b13153
    Publisher
    ACS Publications
    Journal
    Journal of the American Chemical Society
    URI
    http://hdl.handle.net/10545/624098
    DOI
    10.1021/jacs.5b13153
    Additional Links
    https://pubs.acs.org/doi/10.1021/jacs.5b13153
    Type
    Article
    Language
    en
    ISSN
    27863
    EISSN
    15205126
    ae974a485f413a2113503eed53cd6c53
    10.1021/jacs.5b13153
    Scopus Count
    Collections
    School of Human Sciences

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