Authors
Perry, DPaulose Nadappuram, B
Momotenko, D
Voyias, P.D
Page, A
Tripathi, G
Frenguelli, B.G
Unwin, P.R
Affiliation
University of WarwickIssue Date
12/02/2016
Metadata
Show full item recordAbstract
Scanning ion conductance microscopy (SICM) is demonstrated to be a powerful technique for quantitative nanoscale surface charge mapping of living cells. Utilizing a bias modulated (BM) scheme, in which the potential between a quasi-reference counter electrode (QRCE) in an electrolyte-filled nanopipette and a QRCE in bulk solution is modulated, it is shown that both the cell topography and the surface charge present at cellular interfaces can be measured simultaneously at high spatial resolution with dynamic potential measurements. Surface charge is elucidated by probing the properties of the diffuse double layer (DDL) at the cellular interface, and the technique is sensitive at both low-ionic strength and under typical physiological (high-ionic strength) conditions. The combination of experiments that incorporate pixel-level self-referencing (calibration) with a robust theoretical model allows for the analysis of local surface charge variations across cellular interfaces, as demonstrated on two important living systems. First, charge mapping at Zea mays root hairs shows that there is a high negative surface charge at the tip of the cell. Second, it is shown that there are distinct surface charge distributions across the surface of human adipocyte cells, whose role is the storage and regulation of lipids in mammalian systems. These are new features, not previously recognized, and their implications for the functioning of these cells are highlighted.Citation
Perry, D., Paulose Nadappuram, B., Momotenko, D., et al. (2016). 'Surface charge visualization at viable living cells'. Journal of the American Chemical Society, 138(9), pp.3152-3160. DOI: 10.1021/jacs.5b13153Publisher
ACS PublicationsJournal
Journal of the American Chemical SocietyDOI
10.1021/jacs.5b13153Additional Links
https://pubs.acs.org/doi/10.1021/jacs.5b13153Type
ArticleLanguage
enISSN
27863EISSN
15205126ae974a485f413a2113503eed53cd6c53
10.1021/jacs.5b13153