Short-chain fatty acid acetate stimulates adipogenesis and mitochondrial biogenesis via GPR43 in brown adipocytes.
Authors
Hu, JKyrou, I
Tan, B.K
Dimitriadis, G.K
Ramanjaneya, M
Tripathi, G
Patel, V
James, S
Kawan, M
Chen, J
Randeva, H.S
Affiliation
University of WarwickIssue Date
01/05/2016
Metadata
Show full item recordAbstract
Short-chain fatty acids play crucial roles in a range of physiological functions. However, the effects of short-chain fatty acids on brown adipose tissue have not been fully investigated. We examined the role of acetate, a short-chain fatty acid formed by fermentation in the gut, in the regulation of brown adipocyte metabolism. Our results show that acetate up-regulates adipocyte protein 2, peroxisomal proliferator-activated receptor-γ coactivator-1α, and uncoupling protein-1 expression and affects the morphological changes of brown adipocytes during adipogenesis. Moreover, an increase in mitochondrial biogenesis was observed after acetate treatment. Acetate also elicited the activation of ERK and cAMP response element-binding protein, and these responses were sensitive to G(i/o)-type G protein inactivator, Gβγ-subunit inhibitor, phospholipase C inhibitor, and MAPK kinase inhibitor, indicating a role for the G(i/o)βγ/phospholipase C/protein kinase C/MAPK kinase signaling pathway in these responses. These effects of acetate were mimicked by treatment with 4-chloro-α-(1-methylethyl)-N-2-thiazolylbenzeneacetamide, a synthetic G protein-coupled receptor 43 (GPR43) agonist and were impaired in GPR43 knockdown cells. Taken together, our results indicate that acetate may have important physiological roles in brown adipocytes through the activation of GPR43.Citation
Hu, J., Kyrou, I., Tan, B.K., Dimitriadis, G.K., Ramanjaneya, M., Tripathi, G., Patel, V., James, S., Kawan, M., Chen, J. and Randeva, H.S., 2016. Short-chain fatty acid acetate stimulates adipogenesis and mitochondrial biogenesis via GPR43 in brown adipocytes. Endocrinology, 157(5), pp.1881-1894.Publisher
Oxford AcademicJournal
EndocrinologyDOI
10.1210/en.2015-1944Additional Links
https://academic.oup.com/endo/article/157/5/1881/2422634Type
ArticleLanguage
enISSN
137227EISSN
19457170ae974a485f413a2113503eed53cd6c53
10.1210/en.2015-1944