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dc.contributor.authorDuch, Clara Esucdero*
dc.contributor.authorWilliams, Richard AJ*
dc.contributor.authorTimm, Robert M*
dc.contributor.authorPerez-Tris, Javier*
dc.contributor.authorBenitez, Laura*
dc.date.accessioned2019-02-14T16:40:18Z
dc.date.available2019-02-14T16:40:18Z
dc.date.issued2015-07-06
dc.identifier.citationDuch, C.E., Williams, R.A., Timm, R.M., Perez-Tris, J. and Benitez, L., (2015). 'A century of Shope papillomavirus in museum rabbit specimens'. PloS one, 10(7), pp. 1-16. DOI 10.1371/journal.pone.0132172en_US
dc.identifier.urihttp://hdl.handle.net/10545/623498
dc.description.abstractSylvilagus floridanus Papillomavirus (SfPV) causes growth of large horn-like tumors on rabbits. SfPV was described in cottontail rabbits (probably Sylvilagus floridanus) from Kansa and Iowa by Richard Shope in 1933, and detected in S. audubonii in 2011. It is known almost exclusively from the US Midwest. We explored the University of Kansas Natural History Museum for historical museum specimens infected with SfPV, using molecular techniques, to assess if additional wild species host SfPV, and whether SfPV occurs throughout the host range, or just in the Midwest. Secondary aims were to detect distinct strains, and evidence for strain spatio-temporal specificity. We found 20 of 1395 rabbits in the KU collection SfPV symptomatic. Three of 17 lagomorph species (S. nuttallii, and the two known hosts) were symptomatic, while Brachylagus, Lepus and eight additional Sylvilagus species were not. 13 symptomatic individuals were positive by molecular testing, including the first S. nuttallii detection. Prevalence of symptomatic individuals was significantly higher in Sylvilagus (1.8%) than Lepus. Half of these specimens came from Kansas, though new molecular detections were obtained from Jalisco—Mexico’s first—and Nebraska, Nevada, New Mexico, and Texas, USA. We document the oldest lab-confirmed case (Kansas, 1915), predating Shope’s first case. SfPV amplification was possible from 63.2% of symptomatic museum specimens. Using multiple methodologies, rolling circle amplification and, multiple isothermal displacement amplification in addition to PCR, greatly improved detection rates. Short sequences were obtained from six individuals for two genes. L1 gene sequences were identical to all previously detected sequences; E7 gene sequences, were more variable, yielding five distinct SfPV1 strains that differing by less than 2% from strains circulating in the Midwest and Mexico, between 1915 and 2005. Our results do not clarify whether strains are host species specific, though they are consistent with SfPV specificity to genus Sylvilagus.en_US
dc.description.sponsorshipCosts of lab work were funded by the Spanish Ministry of Science and Innovation, (Ref CGL2010-15734/BOS) awarded to JPT. RW was supported by the Programa Internacional de Captación de Talento (PICATA) de Moncloa Campus de Excelencia Internacional(http://www.campusmoncloa.es/en/calls/picata.php) while writing the manuscript. The authors thank The One University Open Access Author Fund at The University of Kansas for funding this publication. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.urlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0132172en_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectShope Papillomavirus, Museum specimens, Sylvilagus floridanus papillomavirus, cottontail rabbit papillomavirus, jackalopeen_US
dc.titleA century of Shope Papillomavirus in museum rabbit specimensen_US
dc.typeArticleen_US
dc.identifier.eissn1932-6203
dc.contributor.departmentDepartment of Microbiology III, Faculty of Biological Sciences, Universidad Complutense de Madrid,en_US
dc.contributor.departmentDepartment of Zoology and Physical Anthropology, Faculty of Biological Sciences, Universidad Complutense de Madriden_US
dc.contributor.departmentNatural Sciences, Saint Louis University, Madrid,en_US
dc.contributor.departmentDepartment of Ecology and Evolutionary Biology & Natural History Museum, University of Kansasen_US
dc.identifier.journalPLOS ONEen_US
dcterms.dateAccepted2015-06-10
refterms.dateFOA2019-02-14T16:40:18Z


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