Blue whiting (Micromesistius poutassou) muscle protein hydrolysate with in vitro and in vivo antidiabetic properties
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Authors
Harnedy, Pàdraigín A.Parthsarathy, Vadivel

McLaughlin, Chris M.
O'Keeffe, Martina B.
Allsopp, Philip J.
McSorley, Emeir M.
O'Harte, Finbarr P. M.
Fitzgerald, Richard J.
Issue Date
2017-10-29
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A blue whiting (Micromesistius poutassou) protein hydrolysate generated using Alcalase 2.4L and Flavourzyme 500L and its simulated gastrointestinal digestion (SGID) sample was assessed for antidiabetic potential in vitro and in vivo. In addition to inhibiting dipeptidyl peptidase-IV (DPP–IV), the hydrolysates mediated insulin and glucagon-like peptide-1 (GLP-1) release from BRIN-BD11 and GLUTag cells, respectively. No significant difference was observed in insulinotropic and DPP-IV inhibitory activity following SGID, while GLP-1 secretion increased significantly (p < 0.01). SGID resulted in a significant increase in membrane potential, intracellular calcium and cyclic AMP concentration (p < 0.001) versus a glucose control, indicating that insulin secretion may be mediated by the KATP channel-dependent and the protein kinase A pathways. Additionally, acute (90–120min) and persistent (4h) glucose-lowering effects of the blue whiting hydrolysate were observed in normal healthy mice. These results demonstrate that the blue whiting protein hydrolysate had significant metabolic effects relevant to glucose control in vivo.Citation
Harnedy, PA, Parthsarathy, Vadivel, McLaughlin, CM, O'Keefe, MB, Allsopp, Philip,McSorley, E. M., O'Harte, Finbarr and Fitzgerald, RJ (2017) Blue whiting (Micromesistius poutassou) muscle protein hydrolysate with in vitro and in vivo antidiabetic properties. Journal of Functional Foods, 40 . pp. 137-145.Publisher
ElsevierJournal
Journal of Functional FoodsDOI
10.1016/j.jff.2017.10.045Additional Links
http://uir.ulster.ac.uk/39112/Type
Journal articleISSN
1756-4646ae974a485f413a2113503eed53cd6c53
10.1016/j.jff.2017.10.045