Show simple item record

dc.contributor.authorBarnes, Kate M.
dc.contributor.authorGennard, Dorothy E.
dc.date.accessioned2017-01-13T08:59:22Z
dc.date.available2017-01-13T08:59:22Z
dc.date.issued2013-04-07
dc.identifier.citationBarnes, K. M. and Gennard, D. E. (2013) 'Rearing bacteria and maggots concurrently: a protocol using Lucilia sericata (Diptera: Calliphoridae) as a model species', Applied Entomology and Zoology, 48 (3):247en
dc.identifier.issn00036862
dc.identifier.doi10.1007/s13355-013-0181-7
dc.identifier.urihttp://hdl.handle.net/10545/621245
dc.description.abstractMaggot debridement therapy using live Lucilia sericata (Meigen) larvae is an efficient and cost-effective way to treat chronic wounds. The recent increase in studies to assess the antibacterial properties of L. sericata has created a need for a simple, low-cost, and comprehensible rearing and investigative method for researchers with little or no entomological experience. This paper describes and evaluates a reproducible protocol for sterilising and rearing blowfly larvae utilising two sterile artificial diets (blood–yeast agar and pre-prepared blood agar plates) that is suitable for directly investigating the effect of larvae on microbial growth. Using Lucilia sericata as a model, the results show that larval growth on the pre-prepared blood agar diet is detrimental to larval growth and survival, whereas larval growth and survival on the blood–yeast agar diet are comparable to those of larvae raised on porcine liver. This diet is proposed as a standard for blowfly and bacteria interaction studies investigating clinical microbial strains. Developmental data are provided for L. sericata larvae raised on both sterile and nonsterile diets so that researchers can determine the effect of treatment based on the length of time for larvae to reach the required life stage at 25 ± 2 °C. Information on larval ageing (instars at an average of 1, 2, 3 and 4 days), oviposition times (4–5 days after adult emergence) and adult longevity on the diets (102–116 days) is also given.
dc.description.sponsorshipN/Aen
dc.language.isoenen
dc.publisherSpringeren
dc.relation.urlhttp://link.springer.com/10.1007/s13355-013-0181-7en
dc.rightsArchived with thanks to Applied Entomology and Zoologyen
dc.subjectArtificial dieten
dc.subjectAntimicrobialen
dc.subjectInsectsen
dc.subjectMaggot debridement therapyen
dc.titleRearing bacteria and maggots concurrently: a protocol using Lucilia sericata (Diptera: Calliphoridae) as a model speciesen
dc.typeArticleen
dc.identifier.eissn1347605X
dc.contributor.departmentUniversity of Derbyen
dc.contributor.departmentUniversity of Lincolnen
dc.identifier.journalApplied Entomology and Zoologyen
html.description.abstractMaggot debridement therapy using live Lucilia sericata (Meigen) larvae is an efficient and cost-effective way to treat chronic wounds. The recent increase in studies to assess the antibacterial properties of L. sericata has created a need for a simple, low-cost, and comprehensible rearing and investigative method for researchers with little or no entomological experience. This paper describes and evaluates a reproducible protocol for sterilising and rearing blowfly larvae utilising two sterile artificial diets (blood–yeast agar and pre-prepared blood agar plates) that is suitable for directly investigating the effect of larvae on microbial growth. Using Lucilia sericata as a model, the results show that larval growth on the pre-prepared blood agar diet is detrimental to larval growth and survival, whereas larval growth and survival on the blood–yeast agar diet are comparable to those of larvae raised on porcine liver. This diet is proposed as a standard for blowfly and bacteria interaction studies investigating clinical microbial strains. Developmental data are provided for L. sericata larvae raised on both sterile and nonsterile diets so that researchers can determine the effect of treatment based on the length of time for larvae to reach the required life stage at 25 ± 2 °C. Information on larval ageing (instars at an average of 1, 2, 3 and 4 days), oviposition times (4–5 days after adult emergence) and adult longevity on the diets (102–116 days) is also given.


This item appears in the following Collection(s)

Show simple item record