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    Rearing bacteria and maggots concurrently: a protocol using Lucilia sericata (Diptera: Calliphoridae) as a model species

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    Authors
    Barnes, Kate M.
    Gennard, Dorothy E.
    Affiliation
    University of Derby
    University of Lincoln
    Issue Date
    2013-04-07
    
    Metadata
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    Abstract
    Maggot debridement therapy using live Lucilia sericata (Meigen) larvae is an efficient and cost-effective way to treat chronic wounds. The recent increase in studies to assess the antibacterial properties of L. sericata has created a need for a simple, low-cost, and comprehensible rearing and investigative method for researchers with little or no entomological experience. This paper describes and evaluates a reproducible protocol for sterilising and rearing blowfly larvae utilising two sterile artificial diets (blood–yeast agar and pre-prepared blood agar plates) that is suitable for directly investigating the effect of larvae on microbial growth. Using Lucilia sericata as a model, the results show that larval growth on the pre-prepared blood agar diet is detrimental to larval growth and survival, whereas larval growth and survival on the blood–yeast agar diet are comparable to those of larvae raised on porcine liver. This diet is proposed as a standard for blowfly and bacteria interaction studies investigating clinical microbial strains. Developmental data are provided for L. sericata larvae raised on both sterile and nonsterile diets so that researchers can determine the effect of treatment based on the length of time for larvae to reach the required life stage at 25 ± 2 °C. Information on larval ageing (instars at an average of 1, 2, 3 and 4 days), oviposition times (4–5 days after adult emergence) and adult longevity on the diets (102–116 days) is also given.
    Citation
    Barnes, K. M. and Gennard, D. E. (2013) 'Rearing bacteria and maggots concurrently: a protocol using Lucilia sericata (Diptera: Calliphoridae) as a model species', Applied Entomology and Zoology, 48 (3):247
    Publisher
    Springer
    Journal
    Applied Entomology and Zoology
    URI
    http://hdl.handle.net/10545/621245
    DOI
    10.1007/s13355-013-0181-7
    Additional Links
    http://link.springer.com/10.1007/s13355-013-0181-7
    Type
    Article
    Language
    en
    ISSN
    00036862
    EISSN
    1347605X
    ae974a485f413a2113503eed53cd6c53
    10.1007/s13355-013-0181-7
    Scopus Count
    Collections
    School of Environmental Sciences

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