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dc.contributor.authorLynch, Paul
dc.contributor.authorSouch, Graham
dc.contributor.authorZamecnik, Jiri
dc.contributor.authorHarding, Keith
dc.date.accessioned2016-11-07T11:41:43Z
dc.date.available2016-11-07T11:41:43Z
dc.date.issued2016-10
dc.identifier.citationLynch, P., Souch, G.R., Zámečník, J., Harding, K. (2016) OPTIMIZATION OF WATER CONTENT FOR THE CRYOPRESERVATION OF Allium > sativum IN VITRO CULTURES BY ENCAPSULATION-DEHYDRATION, Cryoletters, 37, 5 September/October 2016en
dc.identifier.issn0143-2044
dc.identifier.urihttp://hdl.handle.net/10545/620697
dc.description.abstractBACKGROUND: There is a general requirement to determine and correlate water content to viability for the standardization of conservation protocols to facilitate effective cryostorage of plant germplasm. OBJECTIVE: This study examined water content as a critical factor to optimize the cryostorage of Allium sativum. MATERIALS AND METHODS: Stem discs were excised from post-harvest, stored bulbs prior to cryopreservation by encapsulation-dehydration and water content was determined gravimetrically. RESULTS: Survival of cryopreserved stem discs was 42.5%, with 22.5% exhibiting shoot regrowth following 6 h desiccation. Gravimetric data demonstrated a correlation between water content corresponding with survival / regrowth from desiccated, cryopreserved stem discs. For encapsulated stem discs a 25% residual moisture and corresponding water content of 0.36 g H2O g-1 d.wt correlated with maximal survival following ~6.5 h of desiccation. CONCLUSION: The data concurs with the literature suggesting the formation of a stable vitrified state and a ‘window’ for optimal survival and regrowth that is between 6 – 10 h desiccation. Further studies using differential scanning calorimetry (DSC) are suggested to substantiate these findings
dc.description.sponsorshipFunding: EU COST Action 871 Cryoplanet; EU Crymcept QLK5CT-2002-01279; J. Keller, IPK, Germany for garlic germplasm; K. Harding for the Visiting Professorship at the University of Derby.en
dc.language.isoenen
dc.publisherCryoLettersen
dc.relation.urlhttp://www.cryoletters.org/Abstracts/vol_37_5_2016.htm#308en
dc.subjectCryopreservationen
dc.subjectGarlicen
dc.subjectSurvivalen
dc.subjectVitrificationen
dc.titleOptimization of water content for the cryopreservation of allium sativum In vitro cultures by encapsulation-dehydrationen
dc.typeArticleen
dc.contributor.departmentUniversity of Derbyen
dc.identifier.journalCryoLettersen
refterms.dateFOA2019-02-28T14:52:16Z
html.description.abstractBACKGROUND: There is a general requirement to determine and correlate water content to viability for the standardization of conservation protocols to facilitate effective cryostorage of plant germplasm. OBJECTIVE: This study examined water content as a critical factor to optimize the cryostorage of Allium sativum. MATERIALS AND METHODS: Stem discs were excised from post-harvest, stored bulbs prior to cryopreservation by encapsulation-dehydration and water content was determined gravimetrically. RESULTS: Survival of cryopreserved stem discs was 42.5%, with 22.5% exhibiting shoot regrowth following 6 h desiccation. Gravimetric data demonstrated a correlation between water content corresponding with survival / regrowth from desiccated, cryopreserved stem discs. For encapsulated stem discs a 25% residual moisture and corresponding water content of 0.36 g H2O g-1 d.wt correlated with maximal survival following ~6.5 h of desiccation. CONCLUSION: The data concurs with the literature suggesting the formation of a stable vitrified state and a ‘window’ for optimal survival and regrowth that is between 6 – 10 h desiccation. Further studies using differential scanning calorimetry (DSC) are suggested to substantiate these findings


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