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    Optimization of water content for the cryopreservation of allium sativum In vitro cultures by encapsulation-dehydration

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    Cryoletters37.5.pp308-317.pdf
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    Authors
    Lynch, Paul cc
    Souch, Graham
    Zamecnik, Jiri
    Harding, Keith
    Affiliation
    University of Derby
    Issue Date
    2016-10
    
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    Abstract
    BACKGROUND: There is a general requirement to determine and correlate water content to viability for the standardization of conservation protocols to facilitate effective cryostorage of plant germplasm. OBJECTIVE: This study examined water content as a critical factor to optimize the cryostorage of Allium sativum. MATERIALS AND METHODS: Stem discs were excised from post-harvest, stored bulbs prior to cryopreservation by encapsulation-dehydration and water content was determined gravimetrically. RESULTS: Survival of cryopreserved stem discs was 42.5%, with 22.5% exhibiting shoot regrowth following 6 h desiccation. Gravimetric data demonstrated a correlation between water content corresponding with survival / regrowth from desiccated, cryopreserved stem discs. For encapsulated stem discs a 25% residual moisture and corresponding water content of 0.36 g H2O g-1 d.wt correlated with maximal survival following ~6.5 h of desiccation. CONCLUSION: The data concurs with the literature suggesting the formation of a stable vitrified state and a ‘window’ for optimal survival and regrowth that is between 6 – 10 h desiccation. Further studies using differential scanning calorimetry (DSC) are suggested to substantiate these findings
    Citation
    Lynch, P., Souch, G.R., Zámečník, J., Harding, K. (2016) OPTIMIZATION OF WATER CONTENT FOR THE CRYOPRESERVATION OF Allium > sativum IN VITRO CULTURES BY ENCAPSULATION-DEHYDRATION, Cryoletters, 37, 5 September/October 2016
    Publisher
    CryoLetters
    Journal
    CryoLetters
    URI
    http://hdl.handle.net/10545/620697
    Additional Links
    http://www.cryoletters.org/Abstracts/vol_37_5_2016.htm#308
    Type
    Article
    Language
    en
    ISSN
    0143-2044
    Collections
    Environmental Sustainability Research Centre

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