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    Microbial communities associated with healthy and White Syndrome-affected Echinopora lamellosa in aquaria and experimental treatment with the antibiotic Ampicillin

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    Authors
    Smith, David
    Leary, Peter
    Craggs, Jamie
    Bythell, John C. cc
    Sweet, Michael J. cc
    Affiliation
    University of Derby
    Issue Date
    2015-03-20
    
    Metadata
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    Abstract
    Prokaryotic and ciliate communities of healthy and aquarium White Syndrome (WS)-affected coral fragments were screened using denaturing gradient gel electrophoresis (DGGE). A significant difference (R =0.907, p < 0.001) in16S rRNA prokaryotic diversity was found between healthy (H), sloughed tissue (ST),WS-affected (WSU) and antibiotic treated(WST) samples. Although 3 Vibrio spp were found in WS-affected samples, two of these species were eliminated following ampicillin treatment, yet lesions continued to advance, suggesting they play a minor or secondary role in the pathogenesis.The third Vibrio spin-creased slightly in relative abundance in diseased samples and was abundant in non-dis-eased samples. Interestingly, a Tenacibaculum sp showed the greatest increase in relative abundance between healthy and WS-affected samples, demonstrating consistently high abundance across all WS-affected and treated samples, suggesting Tenacibaculum sp could be a more likely candidate for pathogenesis in this instance. In contrast to previous studies bacterial abundance did not vary significantly (ANOVA,F2, 6=1.000,p= 0.422) be-tween H, ST,WSU or WST. Antimicrobial activity(assessed on Vibrio harveyi cultures) was limited in both H and WSU samples (8.1% ± 8.2 and8.0% ± 2.5, respectively) and did not differ significantly (Kruskal-Wallis, χ 2 (2) =3.842, p= 0.146). A Philaster sp, a Cohnilembus sp and a Pseudokeronopsis sp. were present in all WS-affected samples, but not in healthy samples. The exact role of ciliates in WS is yet to be determined, but it is proposed that they are at least responsible for the neat lesion boundary observed in the disease.
    Citation
    Smith, D. et al (2015) 'Microbial communities associated with healthy and White Syndrome-affected Echinopora lamellosa in aquaria and experimental treatment with the antibiotic Ampicillin', PLOS ONE, 10 (3):e0121780
    Journal
    PLOS ONE
    URI
    http://hdl.handle.net/10545/583334
    DOI
    10.1371/journal.pone.0121780
    Additional Links
    http://dx.plos.org/10.1371/journal.pone.0121780
    Type
    Article
    Language
    en
    ISSN
    1932-6203
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0121780
    Scopus Count
    Collections
    Environmental Sustainability Research Centre

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