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UK University staff experience high levels of sedentary behaviour during work and leisure timeReducing sedentary behaviours at work is imperative. Before effective strategies can be developed there is a need to understand profiles of activity within particular roles and organisations. This study aimed to determine activity profiles of staff by job title at a UK University. Three-hundred and seventeen participants completed the short form International Physical Activity Questionnaire to determine physical activity profiles. Fifty-one participants also wore a wrist worn GENEActiv accelerometer for seven days and completed a self-report diary denoting work and leisure hours. Twenty-one per cent of respondents were categorised as inactive and achieved 298 ± 178 metabolic equivalent minutes per week (MET-min/week). Those in administrative roles were most sedentary (501 ± 161 minutes/day). Accelerometer data highlighted that sedentary time was identical between job roles (pooled mean 8746 ± 823 counts) and equated to 84 ± 9% of total time. During working hour’s management, professional and specialist job roles had the highest level of sedentary time (2066 ± 416 counts). Time spent undertaking sedentary activities during working hours contributes to reduced overall activity and can impede productivity, performance, and health. Interventions encouraging regular movement and preventing sedentary behaviours at work are therefore required.