• Using the theoretical domains framework to improve access to cervical screening for women with intellectual disabilities

      Whitelegg, Victoria; Elander, James; University of Derby (British Psychological Society, 2020-07-03)
      Regular attendance for screening can prevent most cervical cancers, but women with learning disabilities are potentially at greater risk of developing and dying from cervical cancer because current screening processes and practices create inequitable barriers, restricting their access to screening. In response, an objective of Public Health England’s 2018 ‘Screening Inequalities Strategy’ was to reduce inequalities through ‘evidence-based contributions’ to policy and best practice (Public Health England, 2018b). Health psychologists could contribute to this objective by facilitating collaborative work with cervical screening practitioners using the Theoretical Domains Framework. This enables health psychology evidence and theory, combined with the perceptions and experiences of screening practitioners, to identify relevant barriers and enablers to access, and this information can inform interventions and policy changes to make cervical screening programmes more open and effective for women with learning disabilities.
    • Exposure to contact sports results in maintained performance during experimental pain

      Thornton, C; Sheffield, D; Baird, A; Northumbria University; University of Derby (Elsevier, 2020-06-26)
      During pain, motor performance tends to decline. However, athletes who engage in contact sports are able to maintain performance despite the inherent pain that accompanies participation. This may be the result of being challenged rather than threatened by pain; adaptive coping strategies; habituation to pain; or finding pain less bothersome. This study aimed to measure performance of a novel motor task both in pain and not in pain within experienced contact athletes (n = 40), novice contact athletes (n = 40) and non-contact athletes (n = 40). Challenge and threat perceptions were manipulated during the pain condition and measures of pain tolerance, perception, coping styles and bothersomeness were taken. Results indicated that contact athletes, regardless of experience, were able to maintain their performance during painful stimulation. Non-contact athletes, conversely, performed significantly worse during pain stimulation. In addition, contact athletes tended to be more challenged and the non-contact athletes more threatened within the pain condition. Experienced contact athletes demonstrated higher levels of pain tolerance and direct coping, and reported lower levels of pain bothersomeness and intensity than the other groups. The results suggest that even relatively brief exposure to contact sports may be enough to help maintain performance in pain. Being in a challenged state appears to be an important factor during performance in pain. Moreover, pain tolerance, intensity and bothersomeness may differentiate novice and experienced athletes.PerspectiveExposure to voluntary pain and challenge states are associated with adaptive responses to pain. Motor task performance may be maintained in individuals with more experience of sports-related pain.
    • A theory of challenge and threat states in athletes: a revised conceptualization

      Meijen, Carla; Turner, Martin; Jones, Marc V; Sheffield, David; McCarthy, Paul; St Mary’s University, Twickenham, London; Manchester Metropolitan University; University of Derby; Glasgow Caledonian University (Frontiers, 2020-02-06)
      The Theory of Challenge and Threat States in Athletes (TCTSA) provides a psychophysiological framework for how athletes anticipate motivated performance situations. The purpose of this review is to discuss how research has addressed the 15 predictions made by the TCTSA, to evaluate the mechanisms underpinning the TCTSA in light of the research that has emerged in the last ten years, and to inform a revised TCTSA (TCTSA-R). There was support for many of the 15 predictions in the TCTSA, with two main areas for reflection identified; to understand the physiology of challenge and to re-evaluate the concept of resource appraisals. This re-evaluation informs the TCTSA-R which elucidates the physiological changes, predispositions, and cognitive appraisals that mark challenge and threat states. First, the relative strength of the sympathetic nervous system response is outlined as a determinant of challenge and threat patterns of reactivity and we suggest that oxytocin and neuropeptide Y are also key indicators of an adaptive approach to motivated performance situations and can facilitate a challenge state. Second, although predispositions were acknowledged within the TCTSA, how these may influence challenge and threat states was not specified. In the TCTSA-R it is proposed that one’s propensity to appraise stressors as a challenge that most strongly dictates acute cognitive appraisals. Third, in the TCTSA-R a more parsimonious integration of Lazarusian ideas of cognitive appraisal and challenge and threat is proposed. Given that an athlete can make both challenge and threat primary appraisals and can have both high or low resources compared to perceived demands, a 2x2 bifurcation theory of challenge and threat is proposed. This reflects polychotomy of four parts; high challenge, low challenge, low threat, and high threat. For example, in low threat, an athlete can evince a threat state but still perform well so long as they perceive high resources. Consequently, we propose suggestions for research concerning measurement tools and a reconsideration of resources to include social support. Finally, applied recommendations are made based on adjusting demands and enhancing resources.
    • Does a natural environment enhance the effectiveness of Mindfulness-Based Stress Reduction (MBSR)? Examining the mental health and wellbeing, and nature connectedness benefits

      Jorgensen, Anna; Sheffield, David; Choe, Eun Yeong; University of Sheffield; University of Derby (Elsevier, 2020-06-24)
      This study investigated whether the impacts of a commonly used wellbeing intervention, Mindfulness-Based Stress Reduction (MBSR), are enhanced when combined with the benefits of exposure to a natural environment. Participants (n = 99) were randomly assigned to a weekly one-hour MBSR in one of three different environments (i.e. natural outdoor, built outdoor and indoor environments) over a six-week period. Participants’ wellbeing outcomes and nature connectedness were measured at baseline, during the intervention and at one-week and one-month follow up. The results show that the mental health and wellbeing outcomes of MBSR are greater when it carried out in a natural outdoor environment compared with indoor or built environments. Moreover, participants in the natural outdoor environment showed sustained improvements even after one month from completion of the intervention. This study supports the potential value of natural environments as settings for the enhancement of health care delivery and therapeutic interventions.
    • Simulated natural environments bolster the effectiveness of a mindfulness programme: A comparison with a relaxation-based intervention

      Choe, Eun Yeong; Jorgensen, Anna; Sheffield, David; University of Sheffield; University of Derby (Elsevier, 2019-12-14)
      This study assesses the effectiveness of incorporating the beneficial effects of exposure to nature in a 3-week mindfulness programme. Participants (n = 122) were randomly assigned to one of two intervention groups (mindfulness, relaxation group) under different simulated environmental conditions (two natural, two non-natural environments) during an intervention lasting three weeks. The participants in the mindfulness group were asked to attend a weekly 1-h mindfulness programme. The relaxation group also spent 1 h per week on relaxation activities of their choice (e.g. reading books or magazines). Participants’ wellbeing outcomes and nature connectedness were measured before and after the three-week intervention, and at one-week follow-up. The findings show that the mindfulness programme was more effective when carried out in a natural environment. In addition, the mindfulness group in natural environments continued to improve even after the intervention was completed. This study offers valuable insights into the benefits of combining a wellbeing intervention with exposure to nature.
    • Measuring actions for nature—development and validation of a pro-nature conservation behaviour scale

      Barbett, Lea; Stupple, Edward; Sweet, Michael; Schofield, Malcolm; Richardson, Miles; University of Derby (MDPI, 2020-06-15)
      Scientists have classed the ongoing decline in biodiversity—caused by humans—as a mass extinction. To mitigate the consequences of this extinction, immediate action is of the utmost importance. However, effective ways of promoting pro-nature conservation behaviours to preserve and enhance biodiversity require better understanding and measurement. Thus, a reliable and valid measurement tool is needed. While there are measurement tools for general pro-environmental behaviours, as of yet, no measure of behaviours that specifically promote biodiversity exists. Here, we present such a tool: the Pro-Nature Conservation Behaviour Scale (ProCoBS), a psychometrically validated questionnaire scale measuring active behaviours that specifically support the conservation of biodiversity. An item pool developed through consultation with wildlife and biodiversity experts was subjected to psychometric scale development analyses. Data from 300 participants were used to develop the 18-item ProCoBS long form, as well as an 8-item short form. A latent variable model with four factors (Individual Engagement, Social Engagement, Planting, and Wildlife) was identified. In a second study, a subset of 250 of the original participants answered the questionnaire again, in addition to related psychological constructs. The data were used to assess test–retest reliability and construct validity. Results showed that the scale and its short form were reliable (full scale: α = 0.893, short form: α = 0.825) and valid. In a third study, a representative sample of 1298 adults in the UK completed the short form. Confirmatory Factor Analysis demonstrated a good fit for all factors, indicating that the ProCoBS is a psychometrically robust measure. The ProCoBS provides the definitive, much needed tool for measuring conservation behaviours. This will enhance research and impact practical work in the conservation domain for a sustainable future. A cross-cultural examination of the scale is still needed. View Full-Text
    • The neurophysiological relationship between number anxiety and the EEG gamma-band

      Baker, Ian; University of Derby (Taylor and Francis, 2020-06-11)
      The development of math anxiety is thought to originate at a young age, as a form of number anxiety, but has not been investigated extensively. Research has shown greater levels of EEG gamma-band activity are experienced during threat perception and attentional bias. This has been identified in high math anxious individuals when confronted with math-based tasks, but has not yet been explored for number anxiety specifically. Single-digit numbers and letters were presented to 15 high and 15 low math anxious participants, who were required to observe the stimuli. High math anxious participants displayed significantly greater levels of gamma activity during number observation compared to letter observation. Findings suggest high math anxious individuals may have a threat-related response to observation of simple numerical stimuli. Further behavioural investigations are needed, but high math anxious individuals may display avoidance towards number and math due to a threat response associated with increased gamma activity.
    • Pain and athletes: Contact sport participation and performance in pain

      Sheffield, David; Thornton, C; Jones, M.V.; University of Derby; Northumbria University; Manchester Metropolitan University (Elsevier BV, 2020-03-29)
      This study examined the effect of cold pressor pain on performance in high-contact athletes, low-contact athletes and non-athletes. A three-group between-subjects experimental design was used. Seventy-one participants completed a motor task and a cognitive task of different complexity (easy or hard) both in pain and not in pain. The motor task involved participants throwing a tennis ball at numbered targets in the correct order. In the cognitive task, participants were required to check off the numbers one to twenty-five in the correct order from a grid of randomly ordered numbers. Task difficulty was increased by adding dummy targets (motor task) or extra numbers (cognitive task). Cold pressor pain was rated as less intense by high-contact athletes during both tasks compared to low-contact athletes and non-athletes. High-contact athletes’ performance was not hampered by pain on the motor task, whereas it was in low-contact athletes and non-athletes. However, pain did not hamper performance for any group during the cognitive task. Low-contact and non-athletes did not differ from each other in their pain reports or the degree to which their performance was hampered by pain in either task. This study provides evidence that adaptation to pain through participation in high-contact sports can enhance both pain tolerance generally and motor performance specifically under increases in pain. The mechanisms behind these differences warrant further exploration.
    • Can compassion, happiness and sympathetic concern be differentiated on the basis of facial expression?

      Condliffe, Otto; Maratos, Frances A.; University of Shanghai; University of Derby (Taylor and Francis, 2020-04-11)
      Recent research has demonstrated the importance of positive emotions, and especially compassion, for well-being. Via two investigations, we set out to determine if facial expressions of happiness, “kind” compassion and sympathetic concern can be distinguished, given limitations of previous research. In investigation one, prototypes of the three expressions were analysed for similarities and differences using the facial action coding system (FACS) by two certified independent coders. Results established that each expression comprised distinct FACS units. Thus, in investigation 2, a new photographic stimulus set was developed using a gender/racially balanced group of actors to pose these expressions of “kind” compassion, happiness, sympathetic concern, and the face in a relaxed/neutral pose. 75 participants were then asked to name the FACS generated expressions using not only forced categorical quantitative ratings but, importantly, free response. Results revealed that kind compassionate facial expressions: (i) engendered words associated with contented and affiliative emotions (although, interestingly, not the word “kind”); (ii) were labelled as compassionate significantly more often than any of the other emotional expressions; but (iii) in common with happiness expressions, engendered happiness word groupings and ratings. Findings have implications for understandings of positive emotions, including specificity of expressions and their veridicality.
    • A randomized-controlled pilot trial of an online compassionate mind training intervention to help people with chronic pain avoid analgesic misuse

      Dhokia, Mayoor; Elander, James; Clements, Keith; Gilbert, Paul; University of Derby (American Psychological Association (APA), 2020-04-09)
      Problematic use of prescribed and over-the-counter analgesics is widespread and increasing among people with chronic pain, but the availability of preventative and treatment services is limited. We evaluated a 21-day online intervention based on compassionate mind training in a prospective, randomized-controlled trial. The participants were 73 adults with concerns about their use of analgesics for chronic pain conditions. Participants completed measures of analgesic use, misuse and dependence, plus self-criticism and self-reassurance (self-inadequacy, self-reassurance and self-hate), cognitive impulsivity (negative urgency, lack of perseverance, lack of premeditation, sensation-seeking and positive urgency) and behavioral impulsivity (delay discounting) at baseline, post-intervention and 1-week post-intervention follow-up. Following baseline assessment, participants were randomized to compassionate mind training (CMT; n=38) or relaxation music (RM; n=35), both delivered online. No adverse events or safety issues were reported and high participant retention and exercise completion rates showed that the intervention was acceptable to participants. Repeated measures analysis of variance showed that by comparison with RM, the CMT group had reduced prescription analgesic use (F=6.123, p=0.015), analgesic dependence (F=14.322, p<.001), self-hate (F=12.218, p<0.001), negative urgency (F=7.323, p=0.006) and lack of perseverance (F=7.453, p=0.001) from baseline to post-intervention, and those improvements were maintained at follow-up. The results show that exercises based on CMT principles and techniques and delivered online can reduce analgesic use, risk of analgesic dependence, and some aspects of self-criticism and impulsivity.
    • Using journal alerts to support your continuing professional development

      Bryson, David; University of Derby (Taylor & Francis, 2020-04-06)
      There are many ways to keep up to date with research that affect your role and personal development. You can regularly use PubMed or Scholar to find recent papers using keyword search, you can rely on others to do the work for you with literature reviews, share the job with Journal clubs or using Journal alerts you can have the papers and research you want delivered to your inbox.
    • The use of an e-learning module on return to work advice for physiotherapists - A prospective cohort study.

      Chance-Larsen, Fiona; Chance-Larsen, Kenneth; Divanoglou, Anestis; Baird, Andrew; Manchester University NHS Foundation Trust; University of Central Lancashire; University of Iceland; University of Derby (Taylor and Francis, 2018-06-20)
      Nonspecific low back pain (LBP) can progress to chronic disability and prolonged absence from work. Despite clinical and professional guidelines, physiotherapists often fail to address return to work outcomes. The aim of this exploratory study was to determine whether an e-learning resource tailored to physiotherapy practice could affect physiotherapists’ attitudes and beliefs regarding return to work advice for their patients. Design: A prospective interventional cohort study (pilot). Methods: Participants were recruited via the Chartered Society of Physiotherapy website. Responses on a clinical vignette, the Health Care Providers’ Pain and Impairment Scale (HC-Pairs), and the Behavioral Constructs Questionnaire (BCQ) were collected online at baseline (Q1) and 2-months post-intervention (Q2). Fifty-four physiotherapists completed Q1 and the response rate for Q2 was 44/54 (81%). Changes in the degree of agreement with guidelines indicated that the intervention made an impact on respondents (kappa 0.345; p = 0.003). HC-Pairs and BCQ results showed a nonstatistically significant trend toward the target behavior. There is a need for interventions to improve adherence with advice for return to work following nonspecific LBP. An e-learning tool for physiotherapists on advising patients regarding return to work has potential to positively affect self-reported clinical behavior.
    • Boarfish (Capros aper) protein hydrolysate has potent insulinotropic and GLP‐1 secretory activity in vitro and acute glucose lowering effects in mice

      Parthsarathy, Vadivel; Mclaughlin, Christopher; Harnedy, Padraigin; Allsopp, Phillip; Crowe, William; McSorley, Emeir; FitzGerald, Dick; O'Harte, Finbarr; University of Ulster; University of Limerick (Wiley, 2018-10-16)
      The anti‐diabetic actions of a boarfish protein hydrolysate (BPH) were investigated in cultured cells and mice. A boarfish (Capros aper) muscle protein hydrolysate was generated using the enzymes Alcalase 2.4 L and Flavourzyme 500 L. Furthermore, the BPH was subjected to simulated gastrointestinal digestion (SGID). BPH and SGID samples (0.01–2.5 mg mL−1) were tested in vitro for DPP‐IV inhibition and insulin and GLP‐1 secretory activity from BRIN‐BD11 and GLUTag cells, respectively. The BPH and SGID samples, caused a dose‐dependent increase (4.2 to 5.3‐fold, P < 0.001) in insulin secretion from BRIN‐BD11 cells and inhibited DPP‐IV activity (IC50 1.18 ± 0.04 and 1.21 ± 0.04 mg mL−1), respectively. The SGID sample produced a 1.3‐fold (P < 0.01) increase in GLP‐1 secretion. An oral glucose tolerance test (OGTT) was conducted in healthy mice (n = 8), with or without BPH (50 mg/kg bodyweight). BPH mediated an increase in plasma insulin levels (AUC(0–120 min), P < 0.05) and a consequent reduction in blood glucose concentration (P < 0.01), after OGTT in mice versus controls. The BPH showed potent anti‐diabetic actions in cells and improved glucose tolerance in mice.
    • Long-term treatment with acylated analogues of apelin-13 amide ameliorates diabetes and improves lipid profile of high-fat fed mice.

      Oharte, FPM; Parthsarathy, Vadivel; Hogg, C; Flatt, P; Ulster University (PLoS, 2018-08-29)
      Previous studies have shown that modified apelin analogues exhibited enzyme resistance in plasma and improved circulating half-life compared to apelin-13. This study investigated the antidiabetic effects of chronic administration of stable long acting fatty acid modified apelin analogues, namely, (Lys8GluPAL)apelin-13 amide and pGlu(Lys8GluPAL)apelin-13 amide, in high-fat fed obese-diabetic mice. Male NIH Swiss mice (groups n = 8) were maintained either on a high-fat diet (45% fat) from 8 to 28 weeks old, or control mice were fed a normal diet (10% fat). When diet induced obesity-diabetes was established after high-fat feeding, mice were injected i.p. once daily with apelin analogues, liraglutide (25 nmol/kg) or saline (controls). Administration of (Lys8GluPAL)apelin-13 amide and pGlu(Lys8GluPAL)apelin-13 amide for 28 days significantly reduced food intake and decreased body weight. Non-fasting glucose was reduced (p<0.01 to p<0.001) and plasma insulin concentrations increased (p<0.01 to p<0.001). This was accompanied by enhanced insulin responses (p<0.01 to p<0.001) and significant reductions in glucose excursion after oral (p<0.01) or i.p. (p<0.01) glucose challenges and feeding. Apelin analogues also significantly improved HbA1c (p<0.01), enhanced insulin sensitivity (p<0.01), reduced triglycerides (p<0.001), increased HDL-cholesterol (p<0.01) and decreased LDL-cholesterol (p<0.01), compared to high-fat fed saline treated control mice. Cholesterol levels were decreased (p<0.01) by pGlu(Lys8GluPAL)apelin-13 amide and both apelin treated groups showed improved bone mineral content, reduced fat deposits and increased plasma GLP-1. Daily treatment with liraglutide mirrored many of these changes (not on bone or adipose tissue), but unlike apelin analogues increased plasma amylase. Consumption of O2, production of CO2, respiratory exchange ratio and energy expenditure were improved by apelin analogues. These results indicate that long-term treatment with acylated analogues (Lys8GluPAL)apelin-13 amide and particularly pGlu(Lys8GluPAL)apelin-13 amide resulted in similar or enhanced therapeutic responses to liraglutide in high-fat fed mice. Fatty acid derived apelin analogues represent a new and exciting development in the treatment of obesity-diabetes.
    • Chronic apelin analogue administration is more effective than established incretin therapies for alleviating metabolic dysfunction in diabetic db/db mice.

      O'Harte, Finbarr P M; Parthsarathy, Vadivel; Flatt, Peter R; University of Ulster (Elsevier, 2020-01-03)
      Stable apelin-13 peptide analogues have shown promising acute antidiabetic effects in mice with diet-induced obesity diabetes. Here the efficacy of (pGlu)apelin-13 amide (apelin amide) and the acylated analogue (pGlu)(Lys8GluPAL)apelin-13 amide (apelin FA), were examined following chronic administration in db/db mice, a genetic model of degenerative diabetes. Groups of 9-week old male db/db mice (n = 8) received twice daily injections (09:00 and 17:00 h; i.p.) or saline vehicle, apelin amide, apelin FA, or the established incretin therapies, exendin-4(1-39) or liraglutide, all at 25 nmol/kg body weight for 21 days. Control C57BL/6J mice were given saline twice daily. No changes in body weight or food intake were observed with either apelin or liraglutide treatments, but exendin-4 showed a reduction in cumulative food intake (p < 0.01) compared with saline-treated db/db mice. Apelin analogues and incretin mimetics induced sustained improvements of glycaemia (p < 0.05 to p < 0.001, from day 9-21), lowered HbA1c at 21 days (p < 0.05) and raised plasma insulin concentrations. The treatments also improved OGTT and ipGTT with enhanced insulin responses compared with saline-treated control db/db mice (p < 0.05 to p < 0.001). Apelin amide was superior to incretin mimetics in lowering plasma triglycerides by 34% (p < 0.05). Apelin analogues unlike both incretin mimetics reduced pancreatic α-cell area (p < 0.05 to p < 0.01) and all peptide treatments enhanced pancreatic insulin content (p < 0.05 to p < 0.01). In conclusion, longer-term administration of apelin-13 analogues, induced similar and in some respects more effective metabolic improvements than incretin mimetics in db/db mice, providing a viable alternative approach for counteracting metabolic dysfunction for mild and more degenerative forms of the disease.
    • Motor performance during experimental pain: The influence of exposure to contact sports

      Thornton, Claire; Sheffield, David; Baird, Andrew; University of Derby (Wiley, 2019-01-30)
      Athletes who play contact sports are regularly exposed to pain, yet manage to perform complex tasks without significant decrement. Limited research has suggested that superior pain tolerance in contact athletes may be important in this context and this may be altered via experience of pain. Other psychological variables such as challenge states, pain bothersomeness and coping style may also influence skill execution during pain. Forty experienced contact athletes (>3 years experience), 40 novice contact athletes (<6 months experience) and 40 non-contact athletes performed a motor task both in pain and without pain. During the pain condition, pressure pain was induced and half of each group were given challenge instructions and the other half threat based instructions. Measures of cognitive appraisal, heart rate variability, pain bothersomeness, tolerance and intensity and coping styles were taken. Contact athletes, regardless of experience, performed better during pain compared to the non-contact athletes, this relationship was mediated by pain tolerance and physical bothersomeness. During the threat condition, experience of contact sports moderated performance. Contact athletes were challenged by the pain, regardless of the instructions given, had higher direct coping and found pain less psychologically bothersome. Experienced contact athletes had higher pain tolerance and reported pain as less intense than the other groups. Ahletes who play contact sports may have better coping and adjustment to experimental pain, especially during threatening conditions. Performance during experimental pain is mediated by pain tolerance and physical pain bothersomeness. Athletes with even relatively small amounts of contact sport experience perform better during experimental pain than athletes who play non-contact sports. Experienced contact athletes had higher levels of direct coping and were more challenged and less threatened by pain than non-contact athletes.
    • Acylated apelin-13 amide analogues exhibit enzyme resistance and prolonged insulin releasing, glucose lowering and anorexic properties

      O'Harte, Finbarr P M; Parthsarathy, Vadivel; Hogg, Christopher; Flatt, Peter R; University of Ulster (Elsevier, 2017-10-04)
      The adipokine, apelin has many biological functions but its activity is curtailed by rapid plasma degradation. Fatty acid derived apelin analogues represent a new and exciting avenue for the treatment of obesity-diabetes. This study explores four novel fatty acid modified apelin-13 analogues, namely, Lys8GluPAL)apelin-13 amide, pGlu(Lys8GluPAL)apelin-13 amide, Lys8GluPAL(Tyr13)apelin-13 and Lys8GluPAL(Val13)apelin-13. Fatty acid modification extended the half-life of native apelin-13 to >24 h in vitro. pGlu(Lys8GluPAL)apelin-13 amide was the most potent insulinotropic analogue in BRIN-BD11 cells and isolated islets with maximal stimulatory effects of up to 2.7-fold (p < .001). (Lys8GluPAL)apelin-13 amide (1.9-fold) and Lys8GluPAL(Tyr13)apelin-13 (1.7-fold) were less effective, whereas Lys8GluPAL(Val13)apelin-13 had an inhibitory effect on insulin secretion. Similarly, pGlu(Lys8GluPAL)apelin-13 amide was most potent in increasing beta-cell intracellular Ca2+ concentrations (1.8-fold, p < .001) and increasing glucose uptake in 3T3-L1 adipocytes (2.3-fold, p < .01). Persistent biological action was observed with both pGlu(Lys8GluPAL)apelin-13 amide and (Lys8GluPAL)apelin-13 amide significantly reducing blood glucose (39-43%, p < .01) and enhancing insulin secretion (43-56%, p < .001) during glucose tolerance tests in diet-induced obese mice. pGlu(Lys8GluPAL)apelin-13 amide and (Lys8GluPAL)apelin-13 amide also inhibited feeding (28-40%, p < .001), whereas Lys8GluPAL(Val13)apelin-13 increased food intake (8%, p < .05) in mice. These data indicate that novel enzymatically stable analogues of apelin-13 may be suitable for future development as therapeutic agents for obesity-diabetes.
    • Beneficial long-term antidiabetic actions of N- and C-terminally modified analogues of apelin-13 in diet-induced obese diabetic mice

      Parthsarathy, Vadivel; Hogg, Christopher; Flatt, Peter R.; O'Harte, Finbarr P. M.; University of Ulster; School of Biomedical Sciences, SAAD Centre for Pharmacy and Diabetes; University of Ulster; Coleraine Northern Ireland, UK; School of Biomedical Sciences, SAAD Centre for Pharmacy and Diabetes; University of Ulster; Coleraine Northern Ireland, UK; School of Biomedical Sciences, SAAD Centre for Pharmacy and Diabetes; University of Ulster; Coleraine Northern Ireland, UK; School of Biomedical Sciences, SAAD Centre for Pharmacy and Diabetes; University of Ulster; Coleraine Northern Ireland, UK (Wiley, 2017-07-20)
      To investigate the chronic effects of twice-daily administration of stable apelin analogues, apelin-13 amide and pyroglutamyl (pGlu) apelin-13 amide, on metabolic variables in glucose-intolerant and insulin-resistant diet-induced obese mice fed a high-fat diet for 150 days. Groups of mice received twice-daily (9 am and 5 pm) injections of saline vehicle, apelin-13 amide, (pGlu)apelin-13 amide or exendin-4(1-39) for 28 days (all at 25 nmol/kg). Energy intake, body weight, non-fasting blood glucose, plasma insulin, glucose tolerance, metabolic response to feeding and insulin sensitivity, together with pancreatic hormone content and biochemical variables such as lipids and total GLP-1 were monitored. Dual-energy X-ray absorptiometry analysis and indirect calorimetry were also performed. Administration of apelin-13 amide, (pGlu)apelin-13 amide or exendin-4 significantly decreased body weight, food intake and blood glucose and increased plasma insulin compared with high-fat-fed saline-treated controls (P < .05 and P < .001), Additionally, all peptide-treated groups exhibited improved glucose tolerance (oral and intraperitoneal), metabolic responses to feeding and associated insulin secretion. (pGlu)apelin-13 amide also significantly improved glycated haemoglobin and insulin sensitivity after 28 days. Both (pGlu)apelin-13 amide and exendin-4 increased bone mineral content and decreased respiratory exchange ratio, whereas only (pGlu)apelin-13 amide increased energy expenditure. All treatment groups displayed reduced circulating triglycerides and increased glucagon-like peptide-1 concentrations, although only (pGlu)apelin-13 amide significantly reduced LDL cholesterol and total body fat, and increased pancreatic insulin content. These data indicate the therapeutic potential of stable apelin-13 analogues, with effects equivalent to or better than those of exendin-4.
    • National guidelines and your continuing professional development

      Bryson, David; University of Derby (Taylor & Francis, 2020-01-23)
      There are several links between the national guidelines produced by the Institute of Medical Illustrators (IMI), the development of evidence-based practice and continuing professional development (CPD). This includes their development, research and testing in practice, their use either to support the development of best practice or their direct implementation. This paper suggests a number of ways to engage with the guidelines to support your professional learning and CPD.