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The development of an eDNA based detection method for the invasive shrimp Dikerogammarus haemobaphesDikerogammarus haemobaphes is a freshwater gammarid crustacean native to the Ponto-Caspian region. However, the species is rapidly spreading throughout Western Europe and is classed as a highly invasive species. Here we present a novel eDNA assay aimed at detecting D. haemobaphes and demonstrate its suitability with validation steps conducted in-silico (computer simulations), ex-situ (test of specificity using closely related species) and in-situ (within the field). A survey of freshwater systems in the West-Midlands, United Kingdom, highlighted that D. haemobaphes was present in 26 out of the 39 sites assessed. We conclude that eDNA detection for D. haemobaphes is a promising tool for assessing and mapping the presence/distribution of this invasive amphipod.