Paternity analysis of wild-caught females shows that sperm package size and placement influence fertilization success in the bushcricket Pholidoptera griseoaptera

Hdl Handle:
http://hdl.handle.net/10545/621607
Title:
Paternity analysis of wild-caught females shows that sperm package size and placement influence fertilization success in the bushcricket Pholidoptera griseoaptera
Authors:
Parker, Darren James ( 0000-0003-4027-7779 ) ; Zaborowska, Julia; Ritchie, Michael Gordon; Vahed, Karim ( 0000-0001-8350-2775 )
Abstract:
In species where females store sperm, males may try to influence paternity by the strategic placement of sperm within the female’s sperm storage organ. Sperm may be mixed or layered in storage organs and this can influence sperm use beyond a ‘fair raffle’. In some insects, sperm from different matings is packaged into discrete packets (spermatodoses) which retain their integrity in the female’s sperm storage organ (spermatheca), but little is known about how these may influence patterns of sperm use under natural mating conditions in wild populations. We examined the effect of the size and position of spermatodoses within the spermatheca and number of competing ejaculates on sperm use in female Dark bushcrickets (Pholidoptera griseoaptera) that had mated under unmanipulated field conditions. Females were collected near the end of the mating season and seven hypervariable microsatellite loci were used to assign paternity of eggs laid in the laboratory. Females contained a median of 3 spermatodoses (range 1-6) and only 6 of the 36 females contained more than one spermatodose of the same genotype. Both the size and relative placement of the spermatodoses within the spermatheca had a significant effect on paternity, with a bias against smaller spermatodoses and those further from the single entrance/exit of the spermatheca. A higher number of competing males reduced the chances of siring offspring for each male. Hence both spermatodose size and relative placement in the spermatheca influence paternity.
Affiliation:
University of Lausanne; University of St Andrews; University of Derby
Citation:
Parker, D J, Zaborowska, J, Ritchie, G M, Vahed, K (2017) Paternity analysis of wild-caught females shows that sperm package size and placement influence fertilization success in the bushcricket Pholidoptera griseoaptera. Molecular Ecology doi: 10.1111/mec.14089
Publisher:
Wiley
Journal:
Molecular Ecology
Issue Date:
7-Apr-2017
URI:
http://hdl.handle.net/10545/621607
DOI:
10.1111/mec.14089
Additional Links:
http://doi.wiley.com/10.1111/mec.14089
Type:
Article
Language:
en
ISSN:
9621083
Sponsors:
University of Derby QR funding, NERC, Erasmus, Erasmus Plus
Appears in Collections:
Environmental Sustainability Research Centre

Full metadata record

DC FieldValue Language
dc.contributor.authorParker, Darren Jamesen
dc.contributor.authorZaborowska, Juliaen
dc.contributor.authorRitchie, Michael Gordonen
dc.contributor.authorVahed, Karimen
dc.date.accessioned2017-05-10T17:05:14Z-
dc.date.available2017-05-10T17:05:14Z-
dc.date.issued2017-04-07-
dc.identifier.citationParker, D J, Zaborowska, J, Ritchie, G M, Vahed, K (2017) Paternity analysis of wild-caught females shows that sperm package size and placement influence fertilization success in the bushcricket Pholidoptera griseoaptera. Molecular Ecology doi: 10.1111/mec.14089en
dc.identifier.issn9621083-
dc.identifier.doi10.1111/mec.14089-
dc.identifier.urihttp://hdl.handle.net/10545/621607-
dc.description.abstractIn species where females store sperm, males may try to influence paternity by the strategic placement of sperm within the female’s sperm storage organ. Sperm may be mixed or layered in storage organs and this can influence sperm use beyond a ‘fair raffle’. In some insects, sperm from different matings is packaged into discrete packets (spermatodoses) which retain their integrity in the female’s sperm storage organ (spermatheca), but little is known about how these may influence patterns of sperm use under natural mating conditions in wild populations. We examined the effect of the size and position of spermatodoses within the spermatheca and number of competing ejaculates on sperm use in female Dark bushcrickets (Pholidoptera griseoaptera) that had mated under unmanipulated field conditions. Females were collected near the end of the mating season and seven hypervariable microsatellite loci were used to assign paternity of eggs laid in the laboratory. Females contained a median of 3 spermatodoses (range 1-6) and only 6 of the 36 females contained more than one spermatodose of the same genotype. Both the size and relative placement of the spermatodoses within the spermatheca had a significant effect on paternity, with a bias against smaller spermatodoses and those further from the single entrance/exit of the spermatheca. A higher number of competing males reduced the chances of siring offspring for each male. Hence both spermatodose size and relative placement in the spermatheca influence paternity.en
dc.description.sponsorshipUniversity of Derby QR funding, NERC, Erasmus, Erasmus Plusen
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttp://doi.wiley.com/10.1111/mec.14089en
dc.rightsArchived with thanks to Molecular Ecologyen
dc.subjectPolyandryen
dc.subjectSperm competitionen
dc.subjectCryptic female choiceen
dc.subjectPostcopulatory sexual selectionen
dc.subjectSpermatodoseen
dc.titlePaternity analysis of wild-caught females shows that sperm package size and placement influence fertilization success in the bushcricket Pholidoptera griseoapteraen
dc.typeArticleen
dc.contributor.departmentUniversity of Lausanneen
dc.contributor.departmentUniversity of St Andrewsen
dc.contributor.departmentUniversity of Derbyen
dc.identifier.journalMolecular Ecologyen
dc.contributor.institutionCentre for Biological Diversity; University of St Andrews; St Andrews KY16 9TH UK-
dc.contributor.institutionCentre for Biological Diversity; University of St Andrews; St Andrews KY16 9TH UK-
dc.contributor.institutionCentre for Biological Diversity; University of St Andrews; St Andrews KY16 9TH UK-
dc.contributor.institutionEnvironmental Sustainability Research Centre; University of Derby; Kedleston Road Derby DE22 1GB UK-
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