Microbial communities associated with healthy and White Syndrome-affected Echinopora lamellosa in aquaria and experimental treatment with the antibiotic Ampicillin

Hdl Handle:
http://hdl.handle.net/10545/583334
Title:
Microbial communities associated with healthy and White Syndrome-affected Echinopora lamellosa in aquaria and experimental treatment with the antibiotic Ampicillin
Authors:
Smith, David; Leary, Peter; Craggs, Jamie; Bythell, John C. ( 0000-0003-2416-9786 ) ; Sweet, Michael J. ( 0000-0003-4983-8333 )
Abstract:
Prokaryotic and ciliate communities of healthy and aquarium White Syndrome (WS)-affected coral fragments were screened using denaturing gradient gel electrophoresis (DGGE). A significant difference (R =0.907, p < 0.001) in16S rRNA prokaryotic diversity was found between healthy (H), sloughed tissue (ST),WS-affected (WSU) and antibiotic treated(WST) samples. Although 3 Vibrio spp were found in WS-affected samples, two of these species were eliminated following ampicillin treatment, yet lesions continued to advance, suggesting they play a minor or secondary role in the pathogenesis.The third Vibrio spin-creased slightly in relative abundance in diseased samples and was abundant in non-dis-eased samples. Interestingly, a Tenacibaculum sp showed the greatest increase in relative abundance between healthy and WS-affected samples, demonstrating consistently high abundance across all WS-affected and treated samples, suggesting Tenacibaculum sp could be a more likely candidate for pathogenesis in this instance. In contrast to previous studies bacterial abundance did not vary significantly (ANOVA,F2, 6=1.000,p= 0.422) be-tween H, ST,WSU or WST. Antimicrobial activity(assessed on Vibrio harveyi cultures) was limited in both H and WSU samples (8.1% ± 8.2 and8.0% ± 2.5, respectively) and did not differ significantly (Kruskal-Wallis, χ 2 (2) =3.842, p= 0.146). A Philaster sp, a Cohnilembus sp and a Pseudokeronopsis sp. were present in all WS-affected samples, but not in healthy samples. The exact role of ciliates in WS is yet to be determined, but it is proposed that they are at least responsible for the neat lesion boundary observed in the disease.
Affiliation:
University of Derby
Citation:
Smith, D. et al (2015) 'Microbial communities associated with healthy and White Syndrome-affected Echinopora lamellosa in aquaria and experimental treatment with the antibiotic Ampicillin', PLOS ONE, 10 (3):e0121780
Journal:
PLOS ONE
Issue Date:
20-Mar-2015
URI:
http://hdl.handle.net/10545/583334
DOI:
10.1371/journal.pone.0121780
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0121780
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Biological Sciences Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorSmith, Daviden
dc.contributor.authorLeary, Peteren
dc.contributor.authorCraggs, Jamieen
dc.contributor.authorBythell, John C.en
dc.contributor.authorSweet, Michael J.en
dc.date.accessioned2015-12-07T15:01:11Zen
dc.date.available2015-12-07T15:01:11Zen
dc.date.issued2015-03-20en
dc.identifier.citationSmith, D. et al (2015) 'Microbial communities associated with healthy and White Syndrome-affected Echinopora lamellosa in aquaria and experimental treatment with the antibiotic Ampicillin', PLOS ONE, 10 (3):e0121780en
dc.identifier.issn1932-6203en
dc.identifier.doi10.1371/journal.pone.0121780en
dc.identifier.urihttp://hdl.handle.net/10545/583334en
dc.description.abstractProkaryotic and ciliate communities of healthy and aquarium White Syndrome (WS)-affected coral fragments were screened using denaturing gradient gel electrophoresis (DGGE). A significant difference (R =0.907, p < 0.001) in16S rRNA prokaryotic diversity was found between healthy (H), sloughed tissue (ST),WS-affected (WSU) and antibiotic treated(WST) samples. Although 3 Vibrio spp were found in WS-affected samples, two of these species were eliminated following ampicillin treatment, yet lesions continued to advance, suggesting they play a minor or secondary role in the pathogenesis.The third Vibrio spin-creased slightly in relative abundance in diseased samples and was abundant in non-dis-eased samples. Interestingly, a Tenacibaculum sp showed the greatest increase in relative abundance between healthy and WS-affected samples, demonstrating consistently high abundance across all WS-affected and treated samples, suggesting Tenacibaculum sp could be a more likely candidate for pathogenesis in this instance. In contrast to previous studies bacterial abundance did not vary significantly (ANOVA,F2, 6=1.000,p= 0.422) be-tween H, ST,WSU or WST. Antimicrobial activity(assessed on Vibrio harveyi cultures) was limited in both H and WSU samples (8.1% ± 8.2 and8.0% ± 2.5, respectively) and did not differ significantly (Kruskal-Wallis, χ 2 (2) =3.842, p= 0.146). A Philaster sp, a Cohnilembus sp and a Pseudokeronopsis sp. were present in all WS-affected samples, but not in healthy samples. The exact role of ciliates in WS is yet to be determined, but it is proposed that they are at least responsible for the neat lesion boundary observed in the disease.en
dc.language.isoenen
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0121780en
dc.rightsArchived with thanks to PLOS ONEen
dc.subjectCoral reefen
dc.subjectDiseaseen
dc.titleMicrobial communities associated with healthy and White Syndrome-affected Echinopora lamellosa in aquaria and experimental treatment with the antibiotic Ampicillinen
dc.typeArticleen
dc.contributor.departmentUniversity of Derbyen
dc.identifier.journalPLOS ONEen
All Items in UDORA are protected by copyright, with all rights reserved, unless otherwise indicated.