Identification of a bacterial pathogen associated with Porites white patch syndrome in the Western Indian Ocean

Hdl Handle:
http://hdl.handle.net/10545/583331
Title:
Identification of a bacterial pathogen associated with Porites white patch syndrome in the Western Indian Ocean
Authors:
Séré, Mathieu G.; Chabanet, Pascale; Quod, Jean-Pascal; Sweet, Michael J. ( 0000-0003-4983-8333 ) ; Tortosa, Pablo ( 0000-0002-7928-7264 ) ; Schleyer, Michael ( 0000-0002-7578-8168 )
Abstract:
Porites white patch syndrome (PWPS) is a coral disease recently described in the Western Indian Ocean. This study aimed to isolate and identify potential pathogens associated with PWPS utilizing both culture and nonculture screening techniques and inoculation trials. A total of 14 bacterial strains (those dominant in disease lesions, absent or rare in healthy tissues and considered potential pathogens in a previous study) were cultured and used to experimentally inoculate otherwise healthy individuals in an attempt to fulfil Henle–Koch's postulates. However, only one (P180R), identified as closely related (99–100% sequence identity based on 1.4 kb 16S RNA sequence) to Vibrio tubiashii, elicited signs of disease in tank experiments. Following experimental infection (which resulted in a 90% infection rate), the pathogen was also successfully re-isolated from the diseased tissues and re-inoculated in healthy corals colonies, therefore fulfilling the final stages of Henle–Koch's postulates. Finally, we report that PWPS appears to be a temperature-dependent disease, with significantly higher tissue loss (anova: d.f. = 2, F = 39.77, P < 0.01) occurring at 30 °C [1.45 ± 0.85 cm2 per day (mean ± SE)] compared to ambient temperatures of 28 and 26 °C (0.73 ± 0.80 cm2 per day (mean ± SE) and 0.51 ± 0.50 cm2 per day (mean ± SE), respectively).
Affiliation:
University of Derby
Citation:
Sere, M, Tortosa, P, Chabanet, P, Quod, J, Sweet, M, & Schleyer, M 2015, 'Identification of a bacterial pathogen associated with Porites white patch syndrome in the Western Indian Ocean', Molecular Ecology, 17, p. 4570
Publisher:
Wiley
Journal:
Molecular Ecology
Issue Date:
Sep-2015
URI:
http://hdl.handle.net/10545/583331
DOI:
10.1111/mec.13326
Additional Links:
http://doi.wiley.com/10.1111/mec.13326
Type:
Article
Language:
en
Series/Report no.:
Vol. 24; Issue 17
ISSN:
09621083
Appears in Collections:
Biological Sciences Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorSéré, Mathieu G.en
dc.contributor.authorChabanet, Pascaleen
dc.contributor.authorQuod, Jean-Pascalen
dc.contributor.authorSweet, Michael J.en
dc.contributor.authorTortosa, Pabloen
dc.contributor.authorSchleyer, Michaelen
dc.date.accessioned2015-12-07T20:21:58Zen
dc.date.available2015-12-07T20:21:58Zen
dc.date.issued2015-09en
dc.identifier.citationSere, M, Tortosa, P, Chabanet, P, Quod, J, Sweet, M, & Schleyer, M 2015, 'Identification of a bacterial pathogen associated with Porites white patch syndrome in the Western Indian Ocean', Molecular Ecology, 17, p. 4570en
dc.identifier.issn09621083en
dc.identifier.doi10.1111/mec.13326en
dc.identifier.urihttp://hdl.handle.net/10545/583331en
dc.description.abstractPorites white patch syndrome (PWPS) is a coral disease recently described in the Western Indian Ocean. This study aimed to isolate and identify potential pathogens associated with PWPS utilizing both culture and nonculture screening techniques and inoculation trials. A total of 14 bacterial strains (those dominant in disease lesions, absent or rare in healthy tissues and considered potential pathogens in a previous study) were cultured and used to experimentally inoculate otherwise healthy individuals in an attempt to fulfil Henle–Koch's postulates. However, only one (P180R), identified as closely related (99–100% sequence identity based on 1.4 kb 16S RNA sequence) to Vibrio tubiashii, elicited signs of disease in tank experiments. Following experimental infection (which resulted in a 90% infection rate), the pathogen was also successfully re-isolated from the diseased tissues and re-inoculated in healthy corals colonies, therefore fulfilling the final stages of Henle–Koch's postulates. Finally, we report that PWPS appears to be a temperature-dependent disease, with significantly higher tissue loss (anova: d.f. = 2, F = 39.77, P < 0.01) occurring at 30 °C [1.45 ± 0.85 cm2 per day (mean ± SE)] compared to ambient temperatures of 28 and 26 °C (0.73 ± 0.80 cm2 per day (mean ± SE) and 0.51 ± 0.50 cm2 per day (mean ± SE), respectively).en
dc.language.isoenen
dc.publisherWileyen
dc.relation.ispartofseriesVol. 24en
dc.relation.ispartofseriesIssue 17en
dc.relation.urlhttp://doi.wiley.com/10.1111/mec.13326en
dc.rightsArchived with thanks to Molecular Ecologyen
dc.subjectCoral reefen
dc.subjectDiseaseen
dc.subjectSea wateren
dc.subjectOceanen
dc.subjectPhylogenyen
dc.subjectRNAen
dc.titleIdentification of a bacterial pathogen associated with Porites white patch syndrome in the Western Indian Oceanen
dc.typeArticleen
dc.contributor.departmentUniversity of Derbyen
dc.identifier.journalMolecular Ecologyen
dc.contributor.institutionAgence pour la Recherche et la Valorisation Marines (ARVAM); Ste Clotilde Reunion Island Franceen
dc.contributor.institutionUnité Mixte de Recherche “Processus Infectieux en Milieu Insulaire Tropical” (UMR PIMIT); Université de La Réunion; Inserm1187; CNRS9192, IRD249; Plateforme de Recherche CYROI; 2 rue Maxime Rivière 97490 Ste Clotilde Saint Denis Franceen
dc.contributor.institutionIRD - UMR ENTROPIE; Labex CORAIL; CS 41095 97495 Sainte Clotilde Cedex La Réunionen
dc.contributor.institutionAgence pour la Recherche et la Valorisation Marines (ARVAM); Ste Clotilde Reunion Island Franceen
dc.contributor.institutionMolecular Health and Disease Laboratory College of Life and Natural Sciences; University of Derby; Kedleston Road Derby UKen
dc.contributor.institutionOceanographic Research Institute (ORI); Durban KwaZulu-Natal South Africaen
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