Experimental antibiotic treatment identifies potential pathogens of white band disease in the endangered Caribbean coral Acropora cervicornis

Hdl Handle:
http://hdl.handle.net/10545/583310
Title:
Experimental antibiotic treatment identifies potential pathogens of white band disease in the endangered Caribbean coral Acropora cervicornis
Authors:
Sweet, Michael J. ( 0000-0003-4983-8333 ) ; Croquer, Aldo; Bythell, John C. ( 0000-0003-2416-9786 )
Abstract:
Coral diseases have been increasingly reported over the past few decades and are a major contributor to coral decline worldwide. The Caribbean, in particular, has been noted as a hotspot for coral disease, and the aptly named white syndromes have caused the decline of the dominant reef building corals throughout their range. White band disease (WBD) has been implicated in the dramatic loss of Acropora cervicornis and Acropora palmata since the 1970s, resulting in both species being listed as critically endangered on the International Union for Conservation of Nature Red list. The causal agent of WBD remains unknown, although recent studies based on challenge experiments with filtrate from infected hosts concluded that the disease is probably caused by bacteria. Here, we report an experiment using four different antibiotic treatments, targeting different members of the disease-associated microbial community. Two antibiotics, ampicillin and paromomycin, arrested the disease completely, and by comparing with community shifts brought about by treatments that did not arrest the disease, we have identified the likely candidate causal agent or agents of WBD. Our interpretation of the experimental treatments is that one or a combination of up to three specific bacterial types, detected consistently in diseased corals but not detectable in healthy corals, are likely causal agents of WBD. In addition, a histophagous ciliate (Philaster lucinda) identical to that found consistently in association with white syndrome in Indo-Pacific acroporas was also consistently detected in allWBDsamples and absent in healthy coral. Treatment with metronidazole reduced it to below detection limits, but did not arrest the disease. However, the microscopic disease signs changed, suggesting a secondary role in disease causation for this ciliate. In future studies to identify a causal agent ofWBDvia tests of Henle–Koch’s postulates, it will be vital to experimentally control for populations of the other potential pathogens identified in this study.
Affiliation:
University of Derby
Citation:
Sweet, M. J. et al (2014) 'Experimental antibiotic treatment identifies potential pathogens of white band disease in the endangered Caribbean coral Acropora cervicornis' , Proceedings of the Royal Society B: Biological Sciences, 281 (1788):20140094
Journal:
Proceedings of the Royal Society B: Biological Sciences
Issue Date:
18-Jun-2014
URI:
http://hdl.handle.net/10545/583310
DOI:
10.1098/rspb.2014.0094
Additional Links:
http://rspb.royalsocietypublishing.org/cgi/doi/10.1098/rspb.2014.0094
Type:
Article
Language:
en
ISSN:
0962-8452; 1471-2954
Appears in Collections:
Biological Sciences Research Group

Full metadata record

DC FieldValue Language
dc.contributor.authorSweet, Michael J.en
dc.contributor.authorCroquer, Aldoen
dc.contributor.authorBythell, John C.en
dc.date.accessioned2015-12-07T14:54:38Zen
dc.date.available2015-12-07T14:54:38Zen
dc.date.issued2014-06-18en
dc.identifier.citationSweet, M. J. et al (2014) 'Experimental antibiotic treatment identifies potential pathogens of white band disease in the endangered Caribbean coral Acropora cervicornis' , Proceedings of the Royal Society B: Biological Sciences, 281 (1788):20140094en
dc.identifier.issn0962-8452en
dc.identifier.issn1471-2954en
dc.identifier.doi10.1098/rspb.2014.0094en
dc.identifier.urihttp://hdl.handle.net/10545/583310en
dc.description.abstractCoral diseases have been increasingly reported over the past few decades and are a major contributor to coral decline worldwide. The Caribbean, in particular, has been noted as a hotspot for coral disease, and the aptly named white syndromes have caused the decline of the dominant reef building corals throughout their range. White band disease (WBD) has been implicated in the dramatic loss of Acropora cervicornis and Acropora palmata since the 1970s, resulting in both species being listed as critically endangered on the International Union for Conservation of Nature Red list. The causal agent of WBD remains unknown, although recent studies based on challenge experiments with filtrate from infected hosts concluded that the disease is probably caused by bacteria. Here, we report an experiment using four different antibiotic treatments, targeting different members of the disease-associated microbial community. Two antibiotics, ampicillin and paromomycin, arrested the disease completely, and by comparing with community shifts brought about by treatments that did not arrest the disease, we have identified the likely candidate causal agent or agents of WBD. Our interpretation of the experimental treatments is that one or a combination of up to three specific bacterial types, detected consistently in diseased corals but not detectable in healthy corals, are likely causal agents of WBD. In addition, a histophagous ciliate (Philaster lucinda) identical to that found consistently in association with white syndrome in Indo-Pacific acroporas was also consistently detected in allWBDsamples and absent in healthy coral. Treatment with metronidazole reduced it to below detection limits, but did not arrest the disease. However, the microscopic disease signs changed, suggesting a secondary role in disease causation for this ciliate. In future studies to identify a causal agent ofWBDvia tests of Henle–Koch’s postulates, it will be vital to experimentally control for populations of the other potential pathogens identified in this study.en
dc.language.isoenen
dc.relation.urlhttp://rspb.royalsocietypublishing.org/cgi/doi/10.1098/rspb.2014.0094en
dc.rightsArchived with thanks to Proceedings of the Royal Society B: Biological Sciencesen
dc.subjectCoral reefen
dc.subjectDiseaseen
dc.titleExperimental antibiotic treatment identifies potential pathogens of white band disease in the endangered Caribbean coral Acropora cervicornisen
dc.typeArticleen
dc.contributor.departmentUniversity of Derbyen
dc.identifier.journalProceedings of the Royal Society B: Biological Sciencesen
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